Protective effect of ingesting Citrus aurantifolia (lemon) peel against hepatic damage induced by ethanol in mice
DOI:
https://doi.org/10.12873/Keywords:
Citrus aurantifolia, Citrus fruit, Liver disease, Ethanol.Abstract
ABSTRACT
Introduction: Liver diseases in Peru are a major cause of morbidity and mortality. Ethanol consumption increases the risk of developing liver cirrhosis and other diseases.
Objective: To determine the protective effect of ingesting Citrus aurantifolia (lemon) peel against ethanol-induced liver damage in mice.
Materials and methods: An analytical, experimental, cross-sectional, and prospective study was conducted. Thirty-five adult male Mus musculus mice were used. Citrus aurantifolia (lemon) peels were obtained by manual peeling, then oven-dried at 40°C for 72 hours. They were then ground in a mill and subsequently sieved. They were suspended in a colloidal medium of water with 1% cornstarch. The mice were randomly assigned to five groups (n=7) and received the following oral treatments for five days: groups I and II: 1% starch suspension; group III: 100 mg/kg silymarin in 1% starch suspension; group IV: 100 mg/kg lemon peel; and group V: 400 mg/kg lemon peel. Group I received a 5% glucose solution in their water bowl, while groups II-V received 5% ethanol with 5% glucose. They were euthanized on day six.
Results: The groups that received the lemon peel doses had significantly higher GSH levels than group II. Less histological damage was also observed compared to group II; however, lipid peroxidation levels in liver tissue were significantly higher than in group II.
Conclusions: Citrus aurantifolia administration has a hepatoprotective effect, as expressed in GSH and histological markers.
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