Efecto hepatoprotector del consumo de Nostoc commune (cushuro) frente al daño inducido por dietas ricas en sacarosa en ratones
DOI:
https://doi.org/10.12873/441bejaranoPalavras-chave:
Comuna Nostoc, sacarose, dano hepático, hepatoproteção.Resumo
Objetivo: Avaliar o efeito hepatoprotetor do consumo de Nostoc commune (cusshuro) contra os danos induzidos por dietas ricas em sacarose em camundongos. Métodos: O estudo teve abordagem quantitativa, puramente experimental, com grupo controle e pós-teste. Foram utilizados 28 camundongos machos com peso médio de 30 ± 6,2 ge pulverizados com Nostoc commune. Os animais foram divididos em quatro grupos e receberam as seguintes dietas durante 50 dias: Grupo I: dieta A (sacarose 10%), grupo II: dieta B (sacarose 36,5%), grupo III: dieta C (sacarose 36,5%). + cushuro 1%) e grupo IV: dieta D (sacarose 36,5% + cushuro 3%). Por fim, o fígado foi removido e foram realizadas análises bioquímicas e histológicas. A estatística ANOVA foi aplicada para dados simétricos e Kruskall-Wallis para dados assimétricos. Resultados: Nos grupos III e IV observou-se que os níveis de triglicerídeos (p<0,05) diminuíram e melhor conservação também foi observada a nível histológico. Conclusão: O consumo de Nostoc commune (cusshuro) apresenta efeito hepatoprotetor expresso na redução dos triglicerídeos e na preservação a nível histológico contra os danos induzidos por dietas ricas em sacarose em camundongos.
Referências
de Oliveira C, Cotrim H, y Arrese M. "Factores de riesgo de la enfermedad por hígado graso no alcohólico en poblaciones de Latinoamérica: situación actual y perspectivas." Clin Liver Dis (Hoboken). 2019;13(1):5-8.
Younossi Z, Tacke F, Arrese M, et al. Global perspectives on nonalcoholic fatty liver disease and non-alcoholic Steatohepatitis.Hepatology. 2018.
Crovetto M, Uauy R. Evolución del gasto en alimentos procesados en la población del Gran Santiago en los últimos 20 años. Rev Med Chil. 2012;140(3):305-12.
Organizacion Mundial de la Salud/ Organizacion Panamericana de la Salud. Alimentos y bebidas ultraprocesados en América Latina: tendencias, efecto sobre la obesidad e implicaciones para las políticas públicas; 2015.
Anania C, Perla FM, Olivero F, Pacifico L, Chiesa C. Dieta mediterránea y enfermedad del hígado graso no alcohólico. World J Gastroenterol. 2018; 24 (19): 2083-2094.
Ferramosca A, Di Giacomo M, Zara V. Antioxidant dietary approach in treatment of fatty liver: New insights and updates. World J Gastroenterol. 2017;23(23):4146-4157.
Matsui K, Nazifi E, Kunita S, Wada N, Matsugo S, & Sakamoto T. Novel glycosylated mycosporine-like amino acids with radical scavenging activity from the cyanobacterium Nostoc commune. Journal of Photochemistry and Photobiology B: Biology. 2011; 105(1): 81-89.
Nazifi E, Wada N, Asano T, et al. Characterization of the chemical diversity of glycosylated mycosporine-like amino acids in the terrestrial cyanobacterium Nostoc commune. Journal of Photochemistry and Photobiology B: Biology. 2015; 142: 154-168.
Ku C, Pham T, Park Y, Kim B, Shin M, Kang I, Lee J. Edible blue-green algae reduce the production of pro-inflammatory cytokines by inhibiting NFκB pathway in macrophages and splenocytes. Biochimica et Biophysica Acta (BBA)-General Subjects. (2013);1830(4): 2981-2988.
Takaichi S, Maoka T, & Mochimaru M. (2009). Unique carotenoids in the terrestrial cyanobacterium Nostoc commune NIES-24: 2-Hydroxymyxol 2′- fucoside, nostoxanthin and canthaxanthin. Current microbiology, 59(4), 413- 419.
Olafsdottir A, Thorlacius G, Omarsdottir S, et al. A heteroglycan from the cyanobacterium Nostoc commune modulates LPS-induced inflammatory cytokine secretion by THP-1 monocytes through phosphorylation of ERK1/2 and Akt. Phytomedicine. 2014; 21(11), 1451-1457.
Park Y, Rasmussen H, Ehlers J, et al. Repression of proinflammatory gene expression by lipid extract of Nostoc commune var sphaeroides Kützing, a blue-green alga, via inhibition of nuclear factor-κB in RAW 264.7 macrophages. Nutrition research. 2018; 28(2), 83-91.
Wang H, Wu S, Liu D. Preparation of polysaccharides from cyanobacteria Nostoc commune and their antioxidant activities. Carbohydrate polymers. 2014; 99: 553-555.
Ku, CS, Yang, Y., Park, Y., Lee, J. Health benefits of blue-green algae: prevention of cardiovascular diseases and non-alcoholic fatty liver disease. Medicinal Food Magazine. 2013; 16 (2): 103-111.
Lozada C. Estudio comparativo de la actividad antioxidante de los polisacáridos extracelulares de Nostoc sphaericum y Nostoc commune. 2018.
Hernández R, Fernández C y Baptista P. Metodología de la Investigación. 6a. edición. Editorial Interamericana. Mc Graw-Hill. 2018.
Olguin M, Posadas M, Revelant G, y cols. Efectos del consumo elevado de fructosa y sacarosa sobre parámetros metabólicos en ratas obesas y diabéticas. Revista chilena de nutrición.2015; 42(2):151-156.
Fuentes F., Mendoza R.., Rosales A, y col. (2010). Guía de manejo y cuidado de animales de laboratorio: ratón.
Li H, Xu J, Liu Y, et al. Antioxidant and moisture-retention activities of the polysaccharide from Nostoc commune. Carbohydrate Polymers. 2011; 83(4): 1821-1827.
Nascimiento T, Cazarin C, Maróstica M, et al. Microalgae carotenoids intake: influence on cholesterol levels, lipid peroxidation and antioxidant enzymes. Food Research International. 2020.
Ku, Chai Siah et al. “Hypolipidemic Effect of a Blue-Green Alga (Nostoc commune) Is Attributed to Its Nonlipid Fraction by Decreasing Intestinal Cholesterol Absorption in C57BL/6J Mice.” Journal of medicinal food 2015; 11(18): 1214-22.
Rasmussen H, Blobaum K, Park Y, Ehlers S, Lu F, Lee J. Lipid extract of Nostoc commune var. sphaeroides Kutzing, a blue-green alga, inhibits the activation of sterol regulatory element binding proteins in HepG2 cells. The Journal of nutrition. 2008; 138(3): 476-481.
Ma P, Huang R, Jiang J, et al. Potential use of C-phycocyanin in nonalcoholic fatty liver disease. Biochemical and Biophysical Research Communications. 2020.
Nagaraj S, Arulmurugan P, Rajaram M, et al. Hepatoprotective and antioxidative effects of C-phycocyanin from Arthrospira maxima SAG 25780 in CCl4-induced hepatic damage rats. Biomedicine & Preventive Nutrition. 2012; 2(2): 81-85
Oriquat A, Ali A, Mahmoud A, Eid M, Hassan R, & Kamel A. Improving hepatic mitochondrial biogenesis as a postulated mechanism for the antidiabetic effect of Spirulina platensis in comparison with metformin. Applied Physiology, Nutrition, and Metabolism, 2019; 44 (4): 357-364.
Itoh T, Tsuzuki R, Tanaka T, et al. Reduced scytonemin isolated from Nostoc commune induces autophagic cell death in human T-lymphoid cell line Jurkat cells. Food and chemical toxicology. 2013, 76-82.
Itoh T, Koketsu M, Yokota N, et al Reduced scytonemin isolated from Nostoc commune suppresses LPS/IFNγ-induced NO production in murine macrophage RAW264 cells by inducing hemeoxygenase-1 expression via the Nrf2/ARE pathway. Food and chemical toxicology. 2014; 69: 330-338.
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