Multi nutrient functional biscuits “Tumiz” improve nutritional status and growth of children; A clinical trial in malnourished rats.
DOI:
https://doi.org/10.12873/451nadimin1Palabras clave:
Nutritional Status, Growth, Oxidation Stress, Functional Biscuits, MalnourishedResumen
Background: The main cause of malnutrition in Indonesia is insufficient nutrient intake, necessitating efforts to supplement nutrients through supplementary feeding. Conversely, Indonesia possesses a variety of local food ingredients that remain underutilized, thus requiring processing to enrich the nutrient content of snacks for combating malnutrition.malnourished Wistar rats.
Methods: Employing a randomized control group pretest-posttest design, male Wistar rats divided into four groups, each comprising 6 samples. These groups included K1: normal rats fed standard food, K-2: malnourished rats given biscuits from the Ministry of Health program, K-3: malnourished rats fed normal food, and K-4: malnourished rats given Tumiz biscuits. The intervention spanned eight weeks, during which body weight, body length, albumin, IGF-1, and MDA levels were measured before and after the intervention.
Results: Significant increases in body weight were observed between pretest-posttest in all groups. The greatest increase in body weight over two months was found in group K4. The albumin levels increased in the rats, there was no statistically significant difference in albumin levels before and after the intervention acrossall groups: K1 (p=0.336), K2 (p=0.297), K3 (p=0.191), and K4 (p=0.466). All intervention groups experienced a significant increase in body length (p<0.05), including groups K1 (p<0.001), K2 (p=0.003), K3 (p<0.001), and K4 (p<0.001). The increase in body length of group K4 did not differ from group K1. IGF-1 levels of Wistar rats significantly increased in group K4 (p=0.006) and group K2 (p=0.026), while groups K1 and K3 experienced a decrease in IGF-1 levels. There was no difference in MDA levels before and after the intervention across all groups.
Conclusion: Tumiz biscuits can increase body weight, body length, and IGF-1 levels but have not been able to increase albumin levels in malnourished Wistar rats. Tumiz biscuits did not reduce MDA levels in malnourished Wistar rats.
Citas
REFERENCES
World Health Organization - WHO. Malnutrition [Internet]. 2024. Available from: https://www.who.int/news-room/fact-sheets/detail/malnutrition
Ministry of Health RI. Nutrition Status Survey of Indonesia (SSGI) 2022 [Internet]. Jakarta; 2023. Available from: https://kesmas.kemkes.go.id/assets/uploads/contents/attachments/09fb5b8ccfdf088080f2521ff0b4374f.pdf
Black, R. E. et al. Maternal and child undernutrition and overweight in low-income and middle-income countries. The Lancet. Lancet. 2013;382(9890):427–51.
Victora, C. G. et al. Maternal and child undernutrition: consequences for adult health and human capital. Lancet. 2010;371(9609):340–57.
DJP B. The origins of the developmental origins theory. J Intern Med. 2007;261:412–7.
Gluckman PD, Hanson MA, Cooper C TK. Effect of in utero and early-life conditions on adult health and disease. N Engl J Med. 2008;359:61–73.
Victora CG, Adair L, Fall C et al. Maternal and child undernutrition: consequences for adult health and human capital. Lancet. 2008;371:340–570.
Osendarp SJ, Baghurst KI, Bryan J, Calvaresi E, Hughes D, Hussaini M, Karyadi SJ, van Klinken JW, van der Meer JW MM. Effect of a 12-mo micronutrient intervention on learning and memory in well-nourished and marginally nourished school-aged children: 2 parallel, randomized, placebo-controlled studies in Australia and Indonesia. Am J Clin Nutr. 2007;85(4):1072–9.
Jang DH, Greenwood-Van Meerveld B, Johnson AC, Cline RE, Said HM KJ. Malnutrition during postnatal development increases oxidative stress and intestinal inflammation in a murine model. Nutr Res. 2018;55:26–35.
Luthfiyah F, Widjajanto E. Serbuk Daun Kelor Memulihkan Kondisi Fisik Gizi Buruk pada Tikus Model Kurang Energi Protein. J Kedokt Brawijaya. 2013;26(3):131–5.
Helmizar H. Development of dadih powder as a complementary food to prevent children from stunting in West Sumatra, Indonesia [Internet]. Vol. 583, IOP Conference Series: Earth and Environmental Science. 2020. Available from: https://api.elsevier.com/content/abstract/scopus_id/85096360113
Godenau D. Food consumption in the Canary Islands: nutritional implications of food imports and local production. BMC Public Health [Internet]. 2022;22(1). Available from: https://api.elsevier.com/content/abstract/scopus_id/85125429226
Nida S. Local regulation as a nutritional improvement solution: Case study of moringa program in west Sumbawa. J Nutr Sci Vitaminol (Tokyo) [Internet]. 2020;66. Available from: https://api.elsevier.com/content/abstract/scopus_id/85101926355
Roy SK. Improved recipes provide better complementary feeding in young children: findings of community-based trials in Bangladesh. Eur J Clin Nutr [Internet]. 2022;76(2):235–43. Available from: https://api.elsevier.com/content/abstract/scopus_id/85114681181
Bustaman S. Potensi ulat sagu dan prospek pemanfaatannya. 2008;27(10).
Anjani G, Achasan M, Adji S, Djamiatun K. Tepung ulat sagu ( Rhyinchophorus ferrugineus ) imunomodulator Nitric Oxide ( NO ) sirkulasi mencit terapi antimalaria standar. J Gizi Indones. 2018;6(2):131–8.
Sani Silwanah. Sago Larvae Flour ( Rhynchophorus Ferruginenus ) Potency As Basic Ingredients Alternative. Universitas Hasanuddin; 2016.
Majeed A. Iron application improves yield, economic returns and grain-Fe concentration of mungbean. PLoS One [Internet]. 2020;15(3). Available from: https://api.elsevier.com/content/abstract/scopus_id/85082453951
Mastropasqua L. Effects of darkness and light spectra on nutrients and pigments in radish, soybean, mung bean and pumpkin sprouts. Antioxidants [Internet]. 2020;9(6):1–12. Available from: https://api.elsevier.com/content/abstract/scopus_id/85090398057
Kaur N. Impact of growing conditions on proximate, mineral, phenolic composition, amino acid profile, and antioxidant properties of black gram, mung bean, and chickpea microgreens. J Food Process Preserv [Internet]. 2022;46(7). Available from: https://api.elsevier.com/content/abstract/scopus_id/85128791055
Baig MA. Mungbean and pumpkin protein isolates as novel ingredients for the development of meat analogs using heat-induced gelation technique. Front Sustain Food Syst [Internet]. 2023;7. Available from: https://api.elsevier.com/content/abstract/scopus_id/85172156033
McKay S. Effects of Dietary Supplementation with Carrot-Derived Rhamnogalacturonan-I (cRG-I) on Accelerated Protective Immune Responses and Quality of Life in Healthy Volunteers Challenged with Rhinovirus in a Randomized Trial. Nutrients [Internet]. 2022;14(20). Available from: https://api.elsevier.com/content/abstract/scopus_id/85140901075
Kiczorowski P. Effect of fermentation of chosen vegetables on the nutrient, mineral, and biocomponent profile in human and animal nutrition. Sci Rep [Internet]. 2022;12(1). Available from: https://api.elsevier.com/content/abstract/scopus_id/85135424026
Khomich LM. Carrot juice nutritional profile. Vopr Pitan [Internet]. 2020;89(1):86–95. Available from: https://api.elsevier.com/content/abstract/scopus_id/85079823712
Nadimin HA. Komposisi biskuit fungsional bergizi. Indonesia: Pangan; IDS000006554, 2023.
Nadimin HA. Pengembangan Jajanan Multi Gizi Berbasis Pangan Lokal Fungsional untuk Pencegahan Malnutrisi pada Anak Balita. 2022;10.
Nadimin AH, Cahyani A. The Effect of Multi-nutrient Flour ( Tumiz ) Substitution on The Acceptance and Levels of Micro Nutrition Nuggets. Media Gizi Mikro Indones [Internet]. 2022;14(1):23–32. Available from: https://doi.org/10.22435/mgmi.v14i1.6225
Herawati DMD. Effect of Eel Biscuit Supplementation on Height of Children with Stunting Aged 36-60 Months: A Pilot Study. J Nutr Metab [Internet]. 2020;2020. Available from: https://api.elsevier.com/content/abstract/scopus_id/85087068298
Pusparini. The effects of multiple-nutrients fortified biscuits and/or psychosocial parenting education intervention programs on anthropometric and cognitive measures of toddlers. J Nutr Sci Vitaminol (Tokyo) [Internet]. 2020;66. Available from: https://api.elsevier.com/content/abstract/scopus_id/85101916754
Adegboye ARA. Potential Use of Edible Insects in Complementary Foods for Children: A Literature Review [Internet]. Vol. 19, International Journal of Environmental Research and Public Health. 2022. Available from: https://api.elsevier.com/content/abstract/scopus_id/85128243271
Ghasemi A, Jeddi S, Kashfi K. the Laboratory Rat: Age and Body Weight Matter. EXCLI J. 2021;20:1431–45.
Xie T, Zaidi H. Age-dependent small-animal internal radiation dosimetry. Mol Imaging. 2013;12(6):364–75.
Shaw R. Use of factorial designs to optimize animal experiments and reduce animal use. ILAR J. 2002;43(4):223–32.
Astawan M, Wresdiyati T, Saragih AM. Evaluation of Protein Nutritional Quality of Tempe and Boiled Soybean Flours by Rats. J Mutu Pangan Indones J Food Qual [Internet]. 2015;2(1):11–7. Available from: http://journal.ipb.ac.id/index.php/jmpi/article/view/27865
Nuraeni T. Kadar Albumin, Hemoglobin, FZat Besi pada Tikus Putih Setelah Pemberian Makanan Enteral Berformulasi Bahan Pangan Lokal. Universitas Sebelas Maret; 2009.
MyBioSource. ELISA Kit For Albumin Organism Species: Rattus norvegicus. MBS2700447 [Internet]. USA; 2023. Available from: https://www.mybiosource.com/rat-elisa-kits/albumin-alb/2700447
MyBioSource. ELISA Kit for Insulin Like Growth Factor 1 (IGF1); Rattus norvegicus (Rat). MBS2700908 [Internet]. USA: Cloud-Clone-corp; 2023. Available from: https://cdn.mybiosource.com/tds/protocol_manuals/800000-9999999/MBS2700908.pdf
MyBioSource. Rat Malondialdehyde (MDA) ELISA Kit Cat No. MBS268427 [Internet]. USA; 2023. Available from: https://cdn.mybiosource.com/tds/protocol_manuals/000000-799999/MBS268427.pdf
Luthfiyah F. Kurang GiziPengaruh Serbuk Daun Kelor Nusa Tenggara Barat Pada Tikus Kurang Gizi. Indones J Hum Nutr. 2013;1–8.
Syam A, Burhan FK, Hadju V, Citrakesumasari C, Akhmar AM. The Effect of Biscuits Made From Pumpkin Seeds Flour on Serum Zinc Levels and Weight in Malnutrition Wistar Rats. Open Access Maced J Med Sci [Internet]. 2020;8:428–33. Available from: https://doi.org/10.3889/oamjms.2020.4402
Purba RB, Sineke J, Laoh JM. Pemberian Biskuit Mama Rani Kepada Balita Berat Badan Kurang. 2023;202–10.
Sarmana, Girsang R, Sembiring A, Yanti MD, Purba, Junita, et al. Pengaruh Pemberian Biskuit Tempe Terhadap Peningkatan Berat Badan Dan Tinggi Badan Pada Balita Sarmana , Girsang R , Sembiring A , Dwi Yanti M , Ayu Yessy Ariescha P , Junita Purba T : Pengaruh Pemberian Biskuit Tempe Terhadap Peningkatan Berat Badan Dan. Inst Kesehat Deli Husada. 2021;4(2):152–8.
Nadimin HA. Laporan Akhir penelitian_Pengembangan jajanan Tumiz untuk pencegahan malnutrisi pada anak balita. Makassar: Poltekkes Kemenkes Makassar;
J. Gordon Betts, Kelly A. Young, James A. Wise, Eddie Johnson, Brandon Poe, Dean H. Kruse, Oksana Korol, Jody E. Johnson, Mark Womble PD. Anatomy and Physiology [Internet]. Houston, Texas: OpenStax; 2013. Available from: https://openstax.org/books/anatomy-and-physiology/pages/1-introduction
Sarbini, Setyaningrum Rahmawaty D. Pre-Clinical Study: Effects of Fe and Zn Fortification in Biscuet Tempeh-Rice Bran On Haemoglobin and Albumin Level of Undernutrition and Anaemia Mice. J Penelit Sains dan Teknol. 2009;10(2):139–47.
Pramudya Kurnia SR. Efek Fortifikasi Fe dan Zn pada Biskuit yang Diolah dari Kombinasi Tempe dan Bekatul untuk Meningkatkan Kadar Albumin Anak balita Kurang Gizi yang Anemia. Surakarta; 2009.
Tarigan N. Asupan Zat Gizi, Hemoglobin, Albumin Dan Berat Badan Anak Balita Gizi Kurang Yang Diberi Cookies Kelor. J Ilm PANNMED (Pharmacist, Anal Nurse, Nutr Midwivery, Environ Dent. 2020;15(2):231–8.
Symond D, Oenzil F, Darwin E, Indrawati Lipoeto N, Studi Ilmu Gizi P, Kesehatan Masyarakat F, et al. Efficacy of Formulated tempe-yam Supplementation on Albumin Level and Weight for Age Z-score for Underweight Children. J Gizi Pangan. 2016;11(1):51–8.
Ahmad A, Madanijah S, Dwiriani CM, Kolopaking R. Effect of nutrition education and multi-nutrient biscuit interventions on nutritional and iron status: A cluster randomized control trial on undernourished children aged 6–23 months in Aceh, Indonesia. J Nutr Sci Vitaminol (Tokyo). 2020;66:S380–90.
Keller U. Nutritional laboratory markers in malnutrition. J Clin Med. 2019;8(6).
Kjaer TW, Grenov B, Yaméogo CW, Fabiansen C, Cichon B, Nielsen NS, et al. Correlates of serum IGF-1 in young children with moderate acute malnutrition : a cross-sectional study in Burkina Faso. Am J Clin Nutr [Internet]. 2021;114(3):965–72. Available from: https://doi.org/10.1093/ajcn/nqab120
Ejlerskov KT, Larnkjaer A, Pedersen D, Ritz C, Mølgaard C MK. IGF-I at 9 and 36 months of age — relations with body composition and diet at 3 years — the SKOT cohort. Growth Horm IGF. Res. 2014;Des;24(6):239–44.
Pusparini, Isdiany N, Tursilowati S. The effects of multiple-nutrients fortified biscuits and/or psychosocial parenting education intervention programs on anthropometric and cognitive measures of toddlers. J Nutr Sci Vitaminol (Tokyo). 2020;66:S443–9.
Nadimin, Nurjaya SSR. The Effect of Local Snacks Enriched with Shell Flour on The Nutritional Status of Stunting Children. Dama Int J Res. 2017;2(7):57–62.
Nadimin RSL. Improving the nutritional value of micro local breast through subtitution of fish flours cabled for prevention of stunting in Sulawesi Selatan. Media Kesehat Politek Kesehat Makassar [Internet]. 2019;XIV(2):152–7. Available from: https://doi.org/10.32382/medkes.v14i2.1021%0A152
N. Marta Díaz-Gómez, Eduardo Doménech, Flora Barroso, Silvia Castells C, Jiménez C and A. The Effect of Zinc Supplementation on Linear Growth , Body Composition , and Growth Factors in Preterm Infants. Pediatrics [Internet]. 2003;111(October 2014):1002–9. Available from: https://doi.org/10.1542/peds.111.5.1002
Christian P, Saijuddin Shaikh, Abu Ahmed Shamim, Sucheta Mehra, Lee Wu MM. Effect of fortified complementary food supplementation on child growth in rural Bangladesh: a cluster-randomized trial. Int J Epidemiol. 2015;44(6):1862–76.
Ferraz IS, Vieira DMC, Ciampo LAD Jr, Ued FDV, Almeida ACF, Jordão AA Jr, Aragon DC, Martinez EZ, Martinelli CE Jr, Nogueira-de-Almeida CAFerraz IS, Vieira DMC, Ciampo LAD Jr, Ued FDV, Almeida ACF, Jordão AA Jr, Aragon DC, Martinez EZ, Martinelli CE Jr N de AC. Vitamin A deficiency and association between serum retinol and IGF-1 concentrations in Brazilian children with Down syndrome. J Pediatr (Rio J). 2022;98(1):76–83.
Suzuki K, Ito Y, Hashimoto S, Kawado M, Inoue T, Ando M, Watanabe Y, Inaba Y, Tajima K, Nakachi K TA. Association of serum retinol and carotenoids with insulin-like growth factors and insulin-like growth factor binding protein-3 among control subjects of a nested case-control study in the Japan Collaborative Cohort Study. Asian Pac J Cancer Prev. 2019;10(Suppl):29–35.
Dijkhuizen MA, Wieringa FT, West CE, Muherdiyantiningsih M. Concurrent micronutrient deficiencies in lactating mothers and their infants in Indonesia. Am J Clin Nutr. 2001;73(4):786–91.
Du Q, Luo ZC, Nuyt AM, Audibert F, Julien P, Wei SQ, et al. Vitamin A and e Nutritional Status in Relation to Leptin, Adiponectin, IGF-I and IGF-II in Early Life - A Birth Cohort Study. Sci Rep [Internet]. 2018;8(1):6–11. Available from: http://dx.doi.org/10.1038/s41598-017-18531-3
Ayala A, Muñoz MF AS. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014;2014(360438).
Young C AC. Interventions for Malnutrition in Older Adults: A Review of Clinical Effectiveness [Internet]. Can Agency Drugs Technol Heal. 2019;Jan(3).
He J. Dietary curcumin supplementation attenuates inflammation, hepatic injury and oxidative damage in a rat model of intra-uterine growth retardation. Br J Nutr [Internet]. 2018;120(5):537–48. Available from: https://api.elsevier.com/content/abstract/scopus_id/85052607616
Sanyoto DD. Potential Combinations of Pasak Bumi (Eurycoma longifolia Jack), Docosahexaenoic Acid, and Seluang Fish (Rasbora spp.) to Improving Oxidative Stress of Rats (Rattus norvegicus) Brain Undernutrition. Open Access Maced J Med Sci [Internet]. 2022;10:25–32. Available from: https://api.elsevier.com/content/abstract/scopus_id/85122864098
Vieira-Rocha MS. Fetal Undernutrition Modifies Vascular RAS Balance Enhancing Oxidative Damage and Contributing to Remodeling. Int J Mol Sci [Internet]. 2022;23(3). Available from: https://api.elsevier.com/content/abstract/scopus_id/85123102757
Lushchak VI, Duszenko M, Gospodaryov D V., Garaschuk O. Oxidative stress and energy metabolism in the brain: Midlife as a turning point. Antioxidants. 2021;10(11):1–19.
Checa J, Aran JM. Reactive oxygen species: Drivers of physiological and pathological processes. J Inflamm Res. 2020;13:1057–73.
Matheus Santos Fernandes, Anderson Apolônio Pedroza, Severina Cássia de Andrade Silva, Maria Daniele Teixeira de Lemos Matheus Santos Fernandes, Anderson Apolônio Pedroza, Severina Cássia de Andrade Silva, Maria Daniele Teixeira de Lemos, Elenilson M. Ber CL. Undernutrition during development modulates endoplasmic reticulum stress genes in the hippocampus of juvenile rats: Involvement of oxidative stress. Brain Res. 2022;1797(15 December 2022,).
Descargas
Publicado
Cómo citar
Número
Sección
Categorías
Licencia
Derechos de autor 2025 Nutrición Clínica y Dietética Hospitalaria
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.
Los lectores pueden utilizar los textos publicados de acuerdo con la definición BOAI (Budapest Open Access Initiative)
