Effect of Helianthus annuus L. (sunflower) seed suspension against ethanol-induced neurotoxicity in mice.

Abstract

Objective: To evaluate the effect of Helianthus annuus L. seed suspensión against ethanol-induced neurotoxicity in mice. Methods: The present experimental design study used sunflower seed suspension (SSG) and 35 male mice. Five groups were formed to which the following treatments were administered for 15 days, orally: groups I and II (water 10mL/kg), group III (vitamin E 20IU/kg), group IV (SSG 200mg/kg) and group V (SSG 600mg/kg). Thirty minutes after treatment, 40% ethanol (1.8g/kg) was administered intraperitoneally, with the exception of group I. Finally, the brain and cerebellar tissue was extracted to perform biochemical and histological analyses. The ANOVA statistical test was applied for parametric data and Kruskall-Wallis for non-parametric data. Results: Group V presented a decrease in lipoperoxidation levels, higher GSH, GSH/GSSG values ​​and protein sulfhydryl groups, compared to group II, (p<0.05). At the histological level, protection of brain and cerebellar tissue was observed in the groups treated with SSG. Conclusion: Ingestion of Helianthus annuus L. seed suspension presented a neuroprotective effect evidenced by a decrease in lipoperoxidation levels, an increase in the levels of protein sulfhydryl groups and glutathione profile, and histological conservation against ethanol-induced toxicity in mice.