Enhancing the Bioactive Profile of Lemon-Based Beverages through Varietal Honey Supplementation: A Multi-Assay Antioxidant Evaluation
DOI:
https://doi.org/10.12873/Palabras clave:
Lemon honey beverages, Antioxidant assays, Blood pressure biomarkersResumen
This study aimed to compare the antioxidant activity of lemon-based beverages formulated with three different types of honey Calliandra, Acacia, and Kelulut using three complementary antioxidant assays: CUPRAC, FRAP, and DPPH. Lemon juice alone served as the control (F0), while the test formulations included F1 (lemon + Calliandra honey), F2 (lemon + Acacia honeydew honey), and F3 (lemon + Kelulut honey). Results showed that F2 exhibited the highest antioxidant capacity in both CUPRAC and FRAP assays, suggesting strong electron-donating capacity under physiological and acidic conditions. Conversely, F1 showed superior scavenging activity in the DPPH assay, indicating higher performance in lipophilic systems. These findings provide in vitro evidence that antioxidant-rich formulations, particularly those containing Acacia honeydew honey, may have potential implications for managing oxidative stress-related conditions such as hypertension. Further in vivo and clinical studies are needed to validate their effects on blood pressure and oxidative stress biomarkers, including ADMA, SDMA, and F2-isoprostanes.
Referencias
1. Serna-Escolano V, Martínez-Romero D, Giménez MJ, Serrano M, García-Martínez S, Valero D, et al. Enhancing Antioxidant Systems by Preharvest Treatments with Methyl Jasmonate and Salicylic Acid Leads to Maintain Lemon Quality During Cold Storage. Food Chem [Internet]. 2021;338(2):128044. Available from: https://doi.org/10.1016/j.foodchem.2020.128044
2. Abdel-Salam OME, Youness ER, Mohammed NA, Morsy SMY, Omara EA, Sleem AA. Citric Acid Effects on Brain and Liver Oxidative Stress in Lipopolysaccharide-Treated Mice. J Med Food [Internet]. 2014;17(5):588–98. Available from: https://www.liebertpub.com/doi/10.1089/jmf.2013.0065
3. Adibelli Z, Dilek M, Akpolat T. Lemon Juice as an Alternative Therapy in Hypertension in Turkey. Int J Cardiol [Internet]. 2009;135(2):e58–9. Available from: http://dx.doi.org/10.1016/j.ijcard.2008.03.085
4. Kato Y, Domoto T, Hiramitsu M, Katagiri T, Sato K, Miyake Y, et al. Effect on Blood Pressure of Daily Lemon Ingestion and Walking. J Nutr Metab [Internet]. 2014;2014(2):1–6. Available from: https://onlinelibrary.wiley.com/doi/epdf/10.1155/2014/912684
5. González I, Maldonado-Agurto R. The Role of Cellular Senescence in Endothelial Dysfunction and Vascular Remodelling in Arteriovenous Fistula Maturation. J Physiol [Internet]. 2025;1–20. Available from: https://physoc.onlinelibrary.wiley.com/doi/10.1113/JP287387
6. Sharma S, Vaidya D, Rana N. Honey as Natural Sweetener in Lemon Ready-to-Serve Drink. Int J Bio-resource Stress Manag [Internet]. 2016;7(2):320–5. Available from: https://www.indianjournals.com/ijor.aspx?target=ijor:ijbsm&volume=7&issue=2&article=022
7. Ahmed S, Sulaiman SA, Baig AA, Ibrahim M, Liaqat S, Fatima S, et al. Honey as a Potential Natural Antioxidant Medicine: An Insight into Its Molecular Mechanisms of Action. Oxid Med Cell Longev [Internet]. 2018;2018(2):1–19. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC5822819/
8. Al-Kafaween MA, Alwahsh M, Mohd Hilmi AB, Abulebdah DH. Physicochemical Characteristics and Bioactive Compounds of Different Types of Honey and Their Biological and Therapeutic Properties: A Comprehensive Review [Internet]. Vol. 12, Antibiotics. 2023. 1–34 p. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC9952753/pdf/antibiotics-12-00337.pdf
9. Dżugan M, Ciszkowicz E, Tomczyk M, Miłek M, Lecka-Szlachta K. Coniferous Honeydew Honey: Antibacterial Activity and Anti-Migration Properties against Breast Cancer Cell Line (MCF-7). Appl Sci [Internet]. 2024;14(2):1–15. Available from: https://www.mdpi.com/2638106
10. Biluca FC, da Silva B, Caon T, Mohr ETB, Vieira GN, Gonzaga LV, et al. Investigation of Phenolic Compounds, Antioxidant and Anti-Inflammatory Activities in Stingless Bee Honey (Meliponinae). Food Res Int [Internet]. 2020;129(November 2019):108756. Available from: https://doi.org/10.1016/j.foodres.2019.108756
11. Ab Wahab SZ, Nik Hussain NH, Zakaria R, Abdul Kadir A, Mohamed N, Tohit NM, et al. Long-term Effects of Honey on Cardiovascular Parameters and Anthropometric Measurements of Postmenopausal Women. Complement Ther Med [Internet]. 2018;41(April):154–60. Available from: https://doi.org/10.1016/j.ctim.2018.08.015
12. Chua LS, Rahaman NLA, Adnan NA, Eddie Tan TT. Antioxidant Activity of Three Honey Samples in Relation with Their Biochemical Components. J Anal Methods Chem [Internet]. 2013;2013:1–8. Available from: https://onlinelibrary.wiley.com/doi/abs/10.1155/2013/313798
13. Özyürek M, Güçlü K, Tütem E, Bakan KS, Erçaǧ E, Esin Çelik S, et al. A Comprehensive Review of CUPRAC Methodology. Anal Methods [Internet]. 2011;3(11):2439–53. Available from: https://pubs.rsc.org/en/content/articlelanding/2011/ay/c1ay05320e
14. Olas B. Honey and its Phenolic Compounds as an Effective Natural Medicine for Cardiovascular Diseases in Humans? Nutrients [Internet]. 2020;12(2):1–14. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC7070389/pdf/nutrients-12-00283.pdf
15. Zhong Y, Shahidi F. Methods for the Assessment of Antioxidant Activity in Foods [Internet]. Handbook of Antioxidants for Food Preservation. Elsevier Ltd; 2015. 287–333 p. Available from: http://dx.doi.org/10.1016/B978-1-78242-089-7.00012-9
16. Šarić G, Vahčić N, Kovačević DB, Putnik P. The Changes of Flavonoids in Honey During Storage. Processes [Internet]. 2020;8(8):1–11. Available from: https://www.mdpi.com/2227-9717/8/8/943
17. Munteanu IG, Apetrei C. Analytical Methods Used in Determining Antioxidant Activity: A review. Int J Mol Sci [Internet]. 2021;22(7):1–30. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC8037236/pdf/ijms-22-03380.pdf
18. Kishore RK, Halim AS, Syazana MSN, Sirajudeen KNS. Tualang Honey Has Higher Phenolic Content and Greater Radical scavenging Activity Compared with other Honey Sources. Nutr Res [Internet]. 2011;31(4):322–5. Available from: http://dx.doi.org/10.1016/j.nutres.2011.03.001
19. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the Total Phenolic, Flavonoid and Proline Contents in Burkina Fasan Honey, as well as Their Radical Scavenging Activity. Food Chem [Internet]. 2005;91(3):571–7. Available from: https://www.sciencedirect.com/science/article/abs/pii/S027153171100039X
20. Minatel IO, Borges CV, Ferreira MI, Gomez HAG, Chen CYO, Lima GPP. Phenolic Compounds: Functional Properties, Impact of Processing and Bioavailability. INTECH [Internet]. 2017;11:13. Available from: https://www.intechopen.com/books/advanced-biometric-technologies/liveness-detection-in-biometrics
21. Uduwana S, Abeynayake N, Wickramasinghe I. Synergistic, Antagonistic, and Additive Effects on The Resultant Antioxidant Activity in Infusions of Green Tea with Bee Honey and Citrus Limonum Extract as Additives. J Agric Food Res [Internet]. 2023;12(February):1–8. Available from: https://www.mdpi.com/2076-3921/9/5/451#B15-antioxidants-09-00451
22. Apak R, Güçlü K, Özyürek M, Bektaşoǧlu B, Bener M. Cupric Ion Reducing Antioxidant Capacity Assay for Food Antioxidants: Vitamins, Polyphenolics, and Flavonoids in Food Extracts [Internet]. Vol. 477, Methods in Molecular Biology. 2008. 163–193 p. Available from: https://link.springer.com/protocol/10.1007/978-1-60327-517-0_14
23. Amran NH, Zaid SSM, Meng GY, Salleh A, Mokhtar MH. Protective Role of Kelulut Honey against Toxicity Effects of Polystyrene Microplastics on Morphology, Hormones, and Sex Steroid Receptor Expression in the Uterus of Rats. Toxics [Internet]. 2023;11(4):1–17. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC10141738/pdf/toxics-11-00324.pdf
24. Tejero J, Shiva S, Gladwin MT. Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation. Physiol Rev [Internet]. 2019;99(1):311–79. Available from: https://journals.physiology.org/doi/epdf/10.1152/physrev.00036.2017
25. Arlouskaya Y, Sawicka A, Głowala M, Giebułtowicz J, Korytowska N, Tałałaj M, et al. Asymmetric Dimethylarginine (ADMA) and Symmetric Dimethylarginine (SDMA) Concentrations in Patients with Obesity and the Risk of Obstructive Sleep Apnea (OSA). J Clin Med [Internet]. 2019;8(6):1–15. Available from: https://www.mdpi.com/484620
26. Ooi TC, Yaacob M, Rajab NF, Shahar S, Sharif R. The Stingless Bee Honey Protects Against Hydrogen Peroxide-Induced Oxidative Damage and Lipopolysaccharide-Induced Inflammation in Vitro. Saudi J Biol Sci [Internet]. 2021;28(5):2987–94. Available from: https://doi.org/10.1016/j.sjbs.2021.02.039
27. Miyake Y, Kuzuya K, Ueno C, Katayama N, Hayakawa T, Tsuge H, et al. Suppressive Effect of Components in Lemon Juice on Blood Pressure in Spontaneously Hypertensive Rats. Food Sci Technol Int Tokyo [Internet]. 1998;4(1):29–32. Available from: https://www.jstage.jst.go.jp/article/fsti9596t9798/4/1/4_1_29/_article/-char/ja/
28. Sun S, Xia G, Pang H, Zhu J, Li L, Zang H. Phytochemical Analysis and Antioxidant Activities of Various Extracts from the Aerial Part of Anemone baicalensis Turcz.: In Vitro and In Vivo Studies. Molecules [Internet]. 2024;29(19):1–30. Available from: https://www.mdpi.com/2975346
29. Sadeer NB, Montesano D, Albrizio S, Zengin G, Mahomoodally MF. The Versatility of Antioxidant Assays in Food Science and Safety—Chemistry, Applications, Strengths, and lLmitations. Antioxidants [Internet]. 2020;9(8):1–39. Available from: https://www.mdpi.com/2076-3921/9/8/709
30. Kedare SB, Singh RP. Genesis and Development of DPPH Method of Antioxidant Assay. J Food Sci Technol [Internet]. 2011;48(4):412–22. Available from: https://pubmed.ncbi.nlm.nih.gov/23572765/
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Derechos de autor 2025 Nutrición Clínica y Dietética Hospitalaria
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