Effect of a diet containing powdered Citrullus lanatus (watermelon) seed on glucose levels and pancreatic tissue in rats with alloxan-induced diabetogenic symptoms
DOI:
https://doi.org/10.12873/Keywords:
citrullus lanatus, Diabetes, Aloxano, Semilla de sandia, hipoglucemiante, PáncreasAbstract
Diabetes mellitus is a chronic disease of high prevalence,
characterized by persistent hyperglycemia and alterations in insulin secretion or action. In response to this issue, the study of medicinal plants with hypoglycemic potential represents an emerging therapeutic alternative. In this context, the seed of Citrullus lanatus (watermelon) has been proposed as a coadjuvant in glycemic control.
Objective: To evaluate the effect of a diet supplemented
with powdered watermelon seed on glucose levels and pancreatic tissue in rats with alloxan-induced diabetes.
Methods: Thirty male Holtzman albino rats were randomly distributed into five groups: negative control (water), metformin (200 mg/kg), and three experimental groups receiving diets supplemented with 1%, 3%, and 9% watermelon seed. The treatment lasted 9 days. Glycemia was measured on days 1, 5, and 9, and pancreatic histology was assessed at the end
of the study.
Results: The group supplemented with 3% seed showed
a significant reduction in glycemia (469 ± 107 mg/dL on day 1 to 232 ± 48 mg/dL on day 9; p<0.05), along with better preservation of pancreatic tissue. The metformin group also showed significant glycemic reduction (p<0.05). The 1% group had a non-significant decrease, while the 9% group showed no improvements. Histological analysis revealed well preserved Langerhans islets and reduced vacuolization in the 3% and metformin groups.
Conclusion: Dietary supplementation with 3% Citrullus
lanatus seed demonstrated a hypoglycemic effect and potential pancreatic protection in diabetic rats, suggesting its possible use as a nutritional adjunct in diabetes management. Further clinical studies are recommended to confirm these effects.
References
1. Clínica Universidad de Navarra. Hiperglucemia: qué es, síntomas, diagnóstico y tratamiento [Internet]. Navarra: CUN; 2022 [citado 20 de noviembre de 2022]. Disponible en: https://www.cun.es/enfermedades-tratamientos/enfermedades/hiperglucemia
2. Mayo Clinic. Hiperglucemia en la diabetes - Síntomas y causas [Internet]. 2022 [citado 20 de noviembre de 2022]. Disponible en: https://www.mayoclinic.org/es-es/diseases-conditions/hyperglycemia/symptoms-causes/syc-20373631
3. Organización Panamericana de la Salud (OPS/OMS). Diabetes [Internet]. Paho.org; 2018 [citado 20 de noviembre de 2022]. Disponible en: https://www.paho.org/es/temas/diabetes
4. Centro Nacional de Epidemiología, Prevención y Control de Enfermedades. CDC Perú notificó más de 32 mil casos de diabetes en todo el país desde el inicio de la pandemia [Internet]. MINSA; 2022. Disponible en: https://www.dge.gob.pe/portalnuevo/informativo/prensa/cdc-peru-notifico-mas-de-32-mil-casos-de-diabetes-en-todo-el-pais-desde-el-inicio-de-la-pandemia/
5. Pal R, Bhadada SK. COVID-19 and diabetes mellitus: An unholy interaction of two pandemics. Diabetes Metab Syndr. 2020;14(4):513–7. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S1871402120301144
6. Ajiboye BO, Shonibare MT, Oyinloye BE. Antidiabetic activity of watermelon (Citrullus lanatus) juice in alloxan-induced diabetic rats. J Diabetes Metab Disord [Internet]. 2020;19(1):343–52. Disponible en: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7270380/
7. Adebayo AO, Alozie I, Chuke-Okeke SO. Evaluating the influence of Citrullus lanatus seed extracts on electrolytes, urea and creatinine in Streptozotocin-induced diabetic albino rats [Internet]. 2020. Disponible en: https://www.infona.pl/resource/bwmeta1.element.ID-c590584b-fbcd-4e00-ae8a-2f8d3dae40e0
8. Ogbeifun H, Peters D, Monanu M. Effect of Aqueous Extract of Citrullus lanatus (Watermelon) Seeds on Alloxan-Induced Diabetic Wistar Rats. Asian J Res Biochem [Internet]. 2020;7(3):30–44. Disponible en: https://journalajrb.com/index.php/AJRB/article/view/30115/56515
9. Hernández Ruiz de Eguilaz M, Martínez de Morentin B, San-Cristóbal R, Pérez Díez S, Navas-Carretero S, et al. Cambios alimentarios y de estilo de vida como estrategia en la prevención del síndrome metabólico y la diabetes mellitus tipo 2: hitos y perspectivas. An Sist Sanit Navar [Internet]. 2016;39(2):269–89. Disponible en: https://scielo.isciii.es/scielo.php?script=sci_arttext&pid=S1137-66272016000200009
10. Arauz E, Cardoze D, Salehji A, Liguas A. Deficiencia de Vitamina B12 relacionada al uso de Metformina. Rev Médico Científica [Internet]. 2020;33:52–63. Disponible en: https://www.revistamedicocientifica.org/index.php/rmc/article/view/585
11. Wilmot EG, Edwardson CL, Achana FA, Davies MJ, Gorely T, Gray LJ, et al. Sedentary time in adults and the association with diabetes, cardiovascular disease and death: systematic review and meta-analysis. Diabetologia [Internet]. 2012;55(11):2895–905. Disponible en: https://link.springer.com/article/10.1007/s00125-012-2677-z
12. Asociación Americana de Diabetes (ADA). Sitio oficial [Internet]. 2022 [citado 20 de noviembre de 2022]. Disponible en: https://diabetes.org/espanol
13. Guía de Práctica Clínica para el Diagnóstico, Tratamiento y Control de la Diabetes Mellitus Tipo 2 en el Primer Nivel de Atención [Internet]. MINSA; Disponible en: http://bvs.minsa.gob.pe/local/MINSA/3466.pdf
14. Deshmukh C, Jain A. Antidiabetic and antihyperlipidemic effects of methanolic extract of Citrullus lanatus seeds in rats. Int J Pharm Pharm Sci [Internet]. 2018;7(10):232–6. Disponible en: https://www.researchgate.net/publication/28286103
15. Beidokhti MN, Jäger AK. Review of antidiabetic fruits, vegetables, beverages, oils and spices commonly consumed in the diet. J Ethnopharmacol [Internet]. 2017;201:26–41. Disponible en: https://www.sciencedirect.com/science/article/abs/pii/S0378874116323054
16. Ajithadas A, Vijayalakshmi K, Karthikeyan V. In vitro antioxidant screening of Citrullus lanatus leaves [Internet]. ResearchGate; 2014. Disponible en: https://www.researchgate.net/publication/304451594
17. Rezq A. Antidiabetic activity and antioxidant role of Watermelon (Citrullus lanatus) peels in Streptozotocin-induced diabetic rats [Internet]. ResearchGate; 2017. Disponible en: https://www.researchgate.net/publication/329885592
18. Organización Mundial de la Salud. Diabetes Mellitus [Internet]. OPS; 2012. Disponible en: https://www3.paho.org/hq/index.php?option=com_content&view=category&id=4475&layout=blog&Itemid=40610&lang=es
19. Brutsaert EF. Diabetes mellitus (DM) [Internet]. Manual MSD para profesionales; 2020. Disponible en: https://www.msdmanuals.com/es-pe/professional/trastornos-endocrinol%C3%B3gicos-y-metab%C3%B3licos/diabetes-mellitus-y-trastornos-del-metabolismo-de-los-hidratos-de-carbono/diabetes-mellitus-dm
20. MedlinePlus. Hiperglucemia - cuidados personales [Internet]. 2022. Disponible en: https://medlineplus.gov/spanish/ency/patientinstructions/000332.htm
21. Asociación Americana de Diabetes. Diagnóstico [Internet]. ADA; 2022 [citado 20 de noviembre de 2022]. Disponible en: https://diabetes.org/diagnostico
22. American Diabetes Association. Pharmacologic Approaches to Glycemic Treatment: Standards of Medical Care in Diabetes—2021. Diabetes Care [Internet]. 2020;44(Suppl 1):S111–24. Disponible en: https://diabetesjournals.org/care/article/44/Supplement_1/S111/31020
23. Zhao F-F, Suhonen R, Koskinen S, Leino-Kilpi H. Theory-based self-management educational interventions on patients with type 2 diabetes: A systematic review and meta-analysis. J Adv Nurs [Internet]. 2016;73(4):812–33. Disponible en: https://onlinelibrary.wiley.com/doi/full/10.1111/jan.1316
24. American Diabetes Association. Summary of Revisions: Standards of Medical Care in Diabetes—2021. Diabetes Care [Internet]. 2020;44(Suppl 1):S4–6. Disponible en: https://diabetesjournals.org/care/article/44/Supplement_1/S4/30968
25. Powers MA, Bardsley JK, Cypress M, Funnell MM, Harms D, Hess-Fischl A, et al. Diabetes self-anagement education and support in adults with type 2 diabetes. Diabetes Care [Internet]. 2020;43(7):1636–49. Disponible en: https://diabetesjournals.org/care/article/43/7/1636/35565
26. American Association of Diabetes Educators. An Effective Model of Diabetes Care and Education: Revising the AADE7 Self-Care Behaviors®. Diabetes Educ [Internet]. 2020. Disponible en: https://journals.sagepub.com/doi/10.1177/0145721719894903
27. Rubino F, Puhl RM, Cummings DE, Eckel RH, Ryan DH, Mechanick JI, et al. Joint international consensus statement for ending stigma of obesity. Nat Med [Internet]. 2020;26(4):485–97. Disponible en: https://www.nature.com/articles/s41591-020-0803-x
28. Al-Azm AM, Mohammad IS, Al-Juaid SW, et al. Luteolin protects pancreatic β cells against apoptosis and preserves function in diabetic models. Phytomedicine. 2024;120:154856. Disponible en: https://pubmed.ncbi.nlm.nih.gov/PMC10385282/
29. Nissar J, Sidiqi US, Hussain DA, Akbar U. Nutritional composition and bioactive potential of watermelon seeds: a pathway to sustainable food and health innovation. Food and Biology. 2025;4:fb-d4fb00335g. Disponible en: https://pubs.rsc.org/content/articlehtml/2025/fb/d4fb00335g
30. Zhou G, Myers R, Li Y, et al. Role of AMP activated protein kinase in the mechanism of metformin action. J Clin Invest. 2001;108(8):1167–74. Disponible en: https://pubmed.ncbi.nlm.nih.gov/pmc209533/
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