Effect of treatment with Mangifera indica L. peel powder on markers of liver damage in ethanol-intoxicated mice
DOI:
https://doi.org/10.12873/Keywords:
Mangifera indica H., liver damage, ethanol, hepatoprotectionAbstract
Introduction: Liver diseases are one of the most important causes of morbidity and mortality in Peru and worldwide, with chronic alcohol consumption being one of the main risk factors for the development of these pathologies. Objective: To determine the effect of Mangifera indica H. (mango) peel powder on ethanol-induced liver toxicity in mice. Materials and methods: A purely experimental approach was used with a post-test and a control group. Thirty-five male albino Mus musculus BALB/c mice, three months old, were treated with Mangifera indica H. peel powder. The mice were randomly assigned to groups. To induce liver damage, 5% ethanol in 5% glucose solution was administered orally in groups II to V for five days. In the afternoon, a 1% starch suspension was administered to the aforementioned groups. Silymarin (70 mg/kg), mango peel (100 mg/kg), and mango peel (900 mg/kg) were used as transport media for groups III, IV, and V, respectively. The mice were fasted for 12 hours before undergoing open incisional laparotomy for liver removal and analysis. Results: Groups IV and V were significantly lower in liver function compared to group II. Also, lower levels of lipid peroxidation (p < 0.05) and SOD/CAT ratio (p < 0.15) were observed, with an increased GSH profile, confirming the findings of the histological analysis. Conclusions: Ingestion of Mangifera indica (mango) peel had a hepatoprotective effect against ethanol toxicity in mice.
References
Asrani SK, Devarbhavi H, Eaton J, Kamath PS. Burden of liver diseases in the world. J Hepatol. 2019;70(1):151–71.
Bustíos C. Mortalidad por enfermedades hepáticas crónicas en el Perú. Rev Gastroenterol Perú. 2020;40(1):35–42.
Lucey MR, Mathurin P, Morgan TR. Alcoholic hepatitis. N Engl J Med. 2009;360(26):2758–69.
Albano E. Alcohol, oxidative stress and free radical damage. Proc Nutr Soc. 2006;65(3):278–90.
Cederbaum AI. CYP2E1—biochemical and toxicological aspects and role in alcohol‐induced liver injury. Mol Aspects Med. 2022;89:101094.
Gao B, Bataller R. Alcoholic liver disease: pathogenesis and new therapeutic targets. Gastroenterology. 2011;141(5):1572–85.
Bansal S, et al. Protective effect of antioxidants against ethanol-induced hepatotoxicity. Toxicol Rep. 2021;8:112–21.
Salomone F, et al. Natural compounds for the treatment of liver diseases: evidence from experimental and clinical studies. Nutrients. 2020;12(1):28.
Karasawa K, Mohan VR. Phenolic composition and hepatoprotective potential of tropical fruits. Food Chem. 2022;393:133427.
He J, Giusti MM. Anthocyanins: natural colorants with health-promoting properties. Annu Rev Food Sci Technol. 2020;11:83–104.
Ajila CM, Naidu KA, Bhat SG, Prasada Rao UJS. Bioactive compounds and antioxidant potential of mango peel extract. Food Chem. 2010;121(4):984–91.
Masibo M, He Q. Major mango polyphenols and their potential significance to human health. Compr Rev Food Sci Food Saf. 2008;7(4):309–19.
Berardini N, et al. Identification of flavonol and xanthone glycosides from mango peels. J Agric Food Chem. 2003;51(17):5006–10.
Rajan S, et al. Protective role of Mangifera indica peel extract on ethanol-induced liver damage in rats. J Food Biochem. 2018;42(5):e12572.
Ghosh A, et al. Mango peel polyphenols: potent natural antioxidants and hepatoprotective agents. Nutr Res Pract. 2019;13(3):242–51.
Nurul SR, et al. Antioxidant and anti-inflammatory activities of mango peel extract. Food Chem. 2020;303:125385.
Singh V, et al. Mango peel extract ameliorates ethanol-induced oxidative stress in rat liver. Phytomedicine. 2021;80:153375.
Rivera D, et al. Mangiferin and oxidative stress modulation: current evidence. Front Nutr. 2023;10:1120659.
Li J, et al. Bioactive compounds from Mangifera indica L. peel: potential therapeutic applications. Food Chem X. 2024;16:100678.
Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. Springer; 1998.
Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol. 1978;52:302–10.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys. 1959;82(1):70–7.
Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for SOD. J Biol Chem. 1972;247(10):3170–5.
Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121–6.
Bancroft JD, Gamble M. Theory and Practice of Histological Techniques. 8th ed. Elsevier; 2019.
Kim H, et al. Antioxidant and anti-inflammatory activities of mango peel extracts. Food Chem. 2020;121(2):429–36.
Chen J, et al. Mangiferin activates Nrf2/ARE pathway and alleviates ethanol-induced hepatic oxidative stress. Food Funct. 2022;13(7):3675–84.
Wang L, et al. The role of Nrf2 signaling in antioxidant defense and hepatoprotection. Redox Biol. 2021;47:102159.
Wu D, Cederbaum AI. Alcohol, oxidative stress, and free radical damage. Alcohol Res Health. 2003;27(4):277–84.
Xie J, et al. Mangiferin enhances antioxidant defense by promoting glutathione synthesis. Biomed Pharmacother. 2021;142:111981.
Published
Issue
Section
Categories
License
Copyright (c) 2025 Nutrición Clínica y Dietética Hospitalaria
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Los lectores pueden utilizar los textos publicados de acuerdo con la definición BOAI (Budapest Open Access Initiative)
How to Cite
