In vitro additive effect of Chlorella sorokiniana in combination with Vincristine on HT-29 colon cancer cells growth inhibition.
Efecto de Microalgas/quimioterapia contra cáncer de colon humano in vitro
DOI:
https://doi.org/10.12873/434hernandezKeywords:
Chlorella sorokiniana, Cancer de colon, VincristinaAbstract
Objective: To evaluate in vitro the effect of the combination of Chlorella sorokiniana with vincristine on HT-29 colon cancer cells.
Material and method: Chlorella sorokininana growth was
constant on day 28 at a temperature of 34 oC ± 3 oC. Chlorella sorokiniana was cultured in López-Chuken medium. HT-29 cells growth inhibition by the microalga alone or in combination with vincristine was evaluated by the colorimetric reduction MTT assay, and analyzed using the SynergyFinder 2.0 software.
Results: The inhibitory effect of Vincristine on HT-29 cells
was 60% from 0.0037µg/mL. Tumor cells growth inhibition by 106 to 108 Chlorella sorokiniana cells ranged from 60% to
80%. The combination of vincristine and Chlorella inhibited
tumor cells growth from 70% to 90%, being the lower concentration of Chlorella the one that showed a better effect in combination with vincristine. The analysis of the results in SynergyFinder showed a score of -0.708, determining an additive effect.
Conclusion: Chlorella sorokiniana has an additive effect in
combination with vincristine against the human colon cancer line HT-29. Supplementation of C. sorokiniana in the diet of patients with colon cancer may improve their treatment and recovery.
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