Green algae Caulerpa racemosa inhibit proliferation and promote apoptosis in human HT-29 colon cancer by suppressing PI3K/AKT pathway.
DOI:
https://doi.org/10.12873/443nurkolisPalabras clave:
Caulerpa racemosa, sea grapes, HT-29, colon cancer, PI3K/AKT, proliferation, apoptosisResumen
Introduction: Colon cancer is a malignancy of the gas
trointestinal which is one of the most common causes of death worldwide. Tumorigenesis involved oncologic pathway such as PI3K/AKT as the common pathway with the challenges is ther apy resistance. Therefore, an alternative colon cancer treat ments is needed that comes from biological resources such as sea grapes.
Objective: This study aims to determine anticancer po
tency of hexane extract from sea grapes (Caulerpa race
mosa) on HT-29 cell line colon cancer based on suppress
ing PI3K/AKT pathway.
Method: The study was conducted in vitro using the ex
tract of Caulerpa racemosa at concentrations of (0, 400, 800,
and 1.200) µg/mL on the HT-29 cell line based on the level
expression of p-akt related to PI3K/AKT pathway, cleaved
caspase 3 related to apoptosis, and viable cells related to proliferation.
Results: The research results was found that the hexane
extract of Caulerpa racemosa decreased the expression
level of p-akt (Kruskal wallis, p=0,027), increased the
cleaved caspase 3 (Kruskal wallis, p=0,016), and decreased the viable cells (Kruskal wallis, 24 and 48 hours: p=0,024
and 0,034).
Conclusion: Caulerpa racemosa hexane extract can inhibit
colon cancer cells growth by suppressing PI3K/AKT pathway
based on inhibition proliferation and induce apoptosis.
Citas
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Derechos de autor 2024 Nutrición Clínica y Dietética Hospitalaria
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