Effect of efficient microorganisms and fruit extracts on the organoleptic and chemical profile of CCN-51 cacao liquor during postharvest processing
DOI:
https://doi.org/10.12873/454vasquez-2Keywords:
cinética fermentativa, calidad organoléptica, modulación sensorial, mejoramiento biotecnológico, innovación poscosechaAbstract
Introduction. Cocoa fermentation is a critical stage for
the development of its chemical and sensory attributes. The incorporation of effective microorganisms (EM) and fruit extracts has emerged as a strategy to enhance the quality of cocoa liquor.
Objectives. To evaluate the combined effect of three EM
levels (0, 40, and 80%) and fruit extracts of banana, yaca,
and passion fruit on the physicochemical and sensory properties of CCN-51 cocoa liquor.
Materials and Methods. A completely randomized bifac
torial design was applied, consisting of 12 treatments and three replications. Fruit extracts were added at 3%, and EM doses were assigned according to treatment. Physicochemical variables (pH, acidity, moisture, ash, and protein) and sensory attributes (color, appearance, aroma, flavor, and aftertaste) were analyzed using a trained panel.
Results. Fruit extracts significantly affected pH, acidity,
moisture, and ash content. EM% had a marked influence on protein levels and some visual parameters. In sensory evaluation, color and appearance showed a significant interaction between factors, while aroma and aftertaste remained stable with slight treatment-dependent variations. Flavor did not present statistical differences but remained within acceptable sensory ranges.
Discussion. The combination of EM and fruit extracts in
duced technological modifications without altering the sensory integrity of the cocoa. The differentiated responses indicate the potential to modulate specific attributes based on extract type and microbial level.
Conclusions. The integrated application of effective mi
croorganisms and fruit extracts offers a viable strategy to enhance the chemical and sensory quality of CCN-51 cocoa liquor, supporting its competitiveness in specialized markets.
References
1. Vásquez L, Alvarado K, Intriago F, Raju N, Prasad R. Banana and apple extracts with efficient microorganisms and their effect on cadmium reduction in cocoa beans (Theobroma cacao L.). Discov Food [Internet]. 2024;4(163):1–13. Available from: https://doi.org/10.1007/s44187-024-00205-5 DOI: https://doi.org/10.1007/s44187-024-00205-5
2. Intriago F, Alvarado K, Vera J, Vásquez L, Tigselema S, Verduga C. Post-harvest quality of cacao (Theobroma cacao L.) for the chocolate industry. In: García L, Naga M, Zambrano F, Aguilar D, editors. Sustainable Cacao Cultivation in Latin America [Internet]. 1st ed. London: Routledge; 2024. p. 270–91. Available from: https://www.taylorfrancis.com/chapters/edit/10.4324/9781003381761-17/post-harvest-quality-cacao-theobroma-cacao-chocolate-industry-frank-intriago-flor-kerly-alvarado-vásquez-jaime-vera-chang-luis-vásquez-cortez-solanyi-tigselema-zambrano-cristhian-verduga DOI: https://doi.org/10.4324/9781003381761-17
3. Vera J, Vásquez L, Alvarado K, Intriago F, Raju M, Radice M. Physical and Organoleptic Evaluation of 12 Cocoa Clones (Theobroma Cacao L.) of National Type, in Cocoa Liquor—a Study from Ecuador. Syst Smart Technol Innov Soc [Internet]. 2024;870:199–211. Available from: 10.1007/978-3-031-51982-6_18 DOI: https://doi.org/10.1007/978-3-031-51982-6_18
4. Vera J, Benavides J, Vásquez L, Alvarado K, Reyes J, Intriago F, et al. Effects of two fermentative methods on cacao (Theobroma cacao L.) Trinitario, induced with Rhizobium japonicum to reduce cadmium. Rev Colomb Investig Agroindustriales [Internet]. 2023;10(1):95–106. Available from: https://doi.org/10.23850/24220582.5460 DOI: https://doi.org/10.23850/24220582.5460
5. Vásquez L, Montoya E, Cadenas L, Plua J, Camacho C, Cobos F, et al. Evaluación físisco química y sensorial del cacao (Theobroma cacao L.) CCN51 fermentado con Saccharomyces cerevisiae y extracto de fruta (Mango manila) en diferentes grados de madurez. Nutr Clin y Diet Hosp. 2025;45(3):117–28. DOI: https://doi.org/10.12873/453vasquez
6. Vásquez L, Erazo C, Vera J, Intriago F. Induction of rhizobium japonicum in the fermentative mass of two varieties of cacao ( Theobroma Cacao L .) as a strategy for the decrease of cadmium. Int J od Heal Sci [Internet]. 2022;6(3):11354–71. Available from: 10.53730/ijhs.v6nS3.8672 DOI: https://doi.org/10.53730/ijhs.v6nS3.8672
7. Torres A, Vera J, Vásquez L, Alvarado K, Intriago F. Extraction of Cocoa Powder for the Preparation of a Drink by Adding Mucilage and Guava. Sarhad J Agric [Internet]. 2023;39(2):10–8. Available from: https://dx.doi.org/10.17582/journal.sja/2023/39/s2.10.18 DOI: https://doi.org/10.17582/journal.sja/2023/39/s2.10.18
8. Vera J, Vásquez L, Alvarado K, Intriago F, Raju M, Radice M. Physical and organoleptic evaluation of 12 cocoa clones ( Theobroma cacao L .) of National type , in Cocoa liquor — a study from Ecuador. Syst Smart Technol Innov Soc. 2024;870:8–15.
9. Intriago F, Cedeño J, Parraga C, Alvarado K, Vásquez L, Revilla K, et al. Induction of effective microorganisms (EM) in the fermenting mass of cacao (Theobroma cacao L.) and their impact on physicochemical and antioxidant characteristics. Biotecnia [Internet]. 2024;26(2422):1–8. Available from: https://doi.org/10.18633/biotecnia.v26.2422 DOI: https://doi.org/10.18633/biotecnia.v26.2422
10. Morales W, Morante J, Herrera R, Ayuso M, Bernalte M. Efecto de la adición de levaduras y enzimas durante la fermentación sobre la calidad fisicoquímica de los granos de cacao fino de aroma. J Agric Food Res. 2024;16(101126):1–43.
11. Viera W, Shinohara T, Samaniego I, Terada N, Sanada A, Ron L, et al. Pulp Mineral Content of Passion Fruit Germplasm Grown in Ecuador and Its Relationship with Fruit Quality Traits. Plants [Internet]. 2022;11(5):1–19. Available from: https://www.mdpi.com/2223-7747/11/5/697#B93-plants-11-00697 DOI: https://doi.org/10.3390/plants11050697
12. Vásquez L, Durazno L, Rivadeneira C, Vera J, Arboleda L, Intriago F. Utilización De extracto de Jackfruit (Artocarpus Heterophyllus) como estategia para mejorar la calidad del grano de cacao. Rev Científica Multidiscip InvestiGo [Internet]. 2023;4(8):95–117. Available from: https://www.revistainvestigo.com/EditorInvestigo/index.php/hm/article/view/67 DOI: https://doi.org/10.56519/g3zy6452
13. Peña M, Ortiz J, Santander F, Lazo M, Caroca R. Physicochemical changes during controlled laboratory fermentation of cocoa (ccn-51) with the inclusion of fruits and on-farm inoculation. Brazilian J Food Technol [Internet]. 2023;26:1–9. Available from: https://www.scielo.br/j/bjft/a/yc7QZvLwpxx8JZS639ycszH/?lang=en DOI: https://doi.org/10.1590/1981-6723.01323
14. Vera J, Vasquez L, Zapata K, Cevallos R. Caracterización morfológica , fisicoquímica y microbiológica del cacao Macambo ( Theobroma bicolor Humb & Bonpl .) en Ecuador. Rev Agrotecnología Amaz [Internet]. 2024;4(2):1–17. Available from: https://doi.org/10.51252/raa.v4i2.657 DOI: https://doi.org/10.51252/raa.v4i2.657
15. Vera J, Radice M, Vásquez L, Intriago F. Perfil químico de 12 clones tipo nacioal de pasta de cacao (Theobroma cacao L.). Univ y Soc [Internet]. 2024;16(1):126–36. Available from: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S2218-36202024000100126
16. Vásquez L, Cevallos C, Uvidia M, Segobia S, Plua J. Evaluación de métodos fermentativos de cacao Nacional y CCN-51, induciendo microorganismos en yute y cajas Rohan para mejora organoleptico. Rev Luna Azul. 2025;(60):168–81. DOI: https://doi.org/10.17151/luaz.2025.60.10
17. Vásquez L, Rodríguez S, Cadena L, PLua J, Camacho C, Cobos F, et al. Aroma, Ciencia y Tradición: Nuevas fronteras en la fermentación del cacao [Internet]. 1st ed. Rodríguez S, Rojas J, editors. Ediciones GESICAP.; 2025. 1–126 p. Available from: https://edicionesgesicap.com/index.php/gesicap/catalog/book/47
18. Iñiguez F, Espiniza X, Galarza E. Use of prbiotics and organic acids as stimulants of the developpment of broilers: review article. Rev Investig en Ciencias Agropecu y Vet [Internet]. 2021;5(14):166–72. Available from: https://revistaalfa.org/index.php/revistaalfa/article/view/115 DOI: https://doi.org/10.33996/revistaalfa.v5i14.107
19. Mbadi C, Matsinkou R, Ngaha W, Assiene J. Formulation of functional cocoa liquor (Theobroma cacao) from raw and processed beans blend: physicochemical, phytochemical, sensory characteristics and in vitro antioxidant activity. Discov Food [Internet]. 2025;5(186):1–16. Available from: https://link.springer.com/article/10.1007/s44187-025-00488-2?utm_source=chatgpt.com DOI: https://doi.org/10.1007/s44187-025-00488-2
20. Calvo A, Botina B, García M, Cardona W, Montenegro A, Criollo J. Dynamics of cocoa fermentation and its effect on quality. Sci Rep [Internet]. 2021;11(16746):1–15. Available from: https://www.nature.com/articles/s41598-021-95703-2 DOI: https://doi.org/10.1038/s41598-021-95703-2
21. González A, García G, Polanía P, López L, Suárez J. Fermentation and its effect on the physicochemical and sensory attributes of cocoa beans in the Colombian Amazon. PLoS One [Internet]. 2024;19(10):1–20. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0306680 DOI: https://doi.org/10.1371/journal.pone.0306680
22. Montoya E. Evaluación fisico química y sensorial del cacao (Theobroma cacao L.) CCN51 fermentado con Saccharomyces cerevisiae y etracto de fruta (Mango manila) en diferentes grados de madurez [Internet]. Universidad Técnica de Babahoyo; 2025. Available from: http://dspace.utb.edu.ec/handle/49000/3447
23. Cevallos C. Evaluación de métodos fermentativos de cacao Nacional y CCN-51 usando yute, cajas Rohan y microorganismos para mejorar propiedades sensoriales [Internet]. Universidad Técnica de Babahoyo; 2025. Available from: https://dspace.utb.edu.ec/handle/49000/18198
24. Quintana L, García A, Rodríguez A, Hoyos N, Alvis A. Impact of Spontaneous Fermentation on the Physicochemical and Sensory Qualities of Cacao. Fermentation [Internet]. 2025;11(7):1–19. Available from: https://www.mdpi.com/2311-5637/11/7/377?utm_source=chatgpt.com DOI: https://doi.org/10.3390/fermentation11070377
25. Díaz C, Luc V. Functional yeast starter cultures for cocoa fermentation. J Appl Microbiol [Internet]. 2022;133(1):39–66. Available from: https://academic.oup.com/jambio/article/133/1/39/6989019 DOI: https://doi.org/10.1111/jam.15312
26. Camu N, González Á, De Winter T, Van Schoor A, De Bruyne K, Vandamme P, et al. Influence of turning and environmental contamination on the dynamics of populations of lactic acid and acetic acid bacteria involved in spontaneous cocoa bean heap fermentation in Ghana. Appl Environ Microbiol [Internet]. 2008;74(1):86–98. Available from: https://journals.asm.org/doi/10.1128/aem.01512-07 DOI: https://doi.org/10.1128/AEM.01512-07
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