Effect of supplementation of an instant mixture based on cañihua, cocoa and bovine blood on the recovery of anemia in wistar rats
DOI:
https://doi.org/10.12873/451paredes-ugarteKeywords:
suplementacion, hierro, anemia, ratasAbstract
Objective: To verify the effect of supplementation of an instant mixture based on cañihua, cocoa and bovine blood for the recovery of anemia in wistar rats.
Material and methods: An experimental study (randomized) was carried out in Wistar rats with an age range of 21-22 days old. The 24 animals were housed in cages (two per cage). All animals lived in light/dark cycles (12 h/12 h) in a constant temperature environment (24o C ± 2o C). Four experimental groups were formed: experimental group 1, experimental group 2, experimental group 3, experimental group 4 and control group. The experimental groups were supplemented with instant mix (cañihua, cocoa powder, bovine blood meal) and the control group without iron supplementation.
Results: The average age of the animals was 20-21 days old. There were significant differences in three experimental groups. In GE1 Hb increased by 40% (effect size -0.87), in GE2 it increased 22% (effect size-0.79) and in GE3 it increased 23% (effect size -0.79). The CG remained with relatively stable Hb values. There were no significant differences in the three experimental groups in Hb levels (p>0.05).
Conclusion: Supplementation with the instant mixture of cañihua, cocoa and bovine blood meal was effective in increasing hemoglobin levels in four weeks, which makes it a proactive strategy to increase hemoglobin levels
References
McLean E, Cogswell M, Egli I, Wojdyla D, de Benoist B. Worldwide prevalence of anaemia, WHO Vitamin and Mineral Nutrition Information System, 1993-2005. Public Health Nutr [Internet]. 2009;12(4):444–54. Disponible en: http://dx.doi.org/10.1017/S1368980008002401
Lopez A, Cacoub P, Macdougall IC, Peyrin-Biroulet L. Iron deficiency anaemia. Lancet [Internet]. 2016;387(10021):907–16. Disponible en: http://dx.doi.org/10.1016/S0140-6736(15)60865-0
De Benoist B, McLean E, Egli I,Cogswell M. Worldwide Prevalence of Anaemia 1993-2005: WHO Global Database on Anaemia World Health Organization.Geneva; 2008
Fishman SM, Christian P, West KP Jr. The role of vitamins in the prevention and control of anaemia. Public Health Nutr [Internet]. 2000;3(2):125–50. Disponible en: http://dx.doi.org/10.1017/s1368980000000173
Williams AM, Brown KH, Allen LH, Dary O, Moorthy D, Suchdev PS. Improving anemia assessment in clinical and public health settings. J Nutr [Internet]. 2023;153:S29–41. Disponible en: http://dx.doi.org/10.1016/j.tjnut.2023.05.032
Loechl CU, Datta-Mitra A, Fenlason L, Green R, Hackl L, Itzkowitz L, et al. Approaches to address the anemia challenge. J Nutr [Internet]. 2023;153:S42–59. Disponible en: http://dx.doi.org/10.1016/j.tjnut.2023.07.017
Abbaspour N, Hurrell R, Kelishadi R. Review on iron and its importance for human health. J Res Med Sci. 2014;19(2):164–74.
Navas-Carretero S, Sarriá B, Pérez-Granados AM, Schoppen S, Izquierdo-Pulido M, Vaquero MP. A comparative study of iron bioavailability from cocoa supplemented with ferric pyrophosphate or ferrous fumarate in rats. Ann Nutr Metab [Internet]. 2007;51(3):204–7. Disponible en: http://dx.doi.org/10.1159/000104138
Tello-Palma E, Choque-Quispe M, Pacheco-Tanaka M, Zamalloa-Cuba W, Valencia-Pacho M, Donaires-Flores T, et al. Effects of microencapsulated and heme iron supplementation on the recovery of hemoglobin levels in iron-depleted rats. Nutr Hosp [Internet]. 2022;39(6):1357–63. Disponible en: http://dx.doi.org/10.20960/nh.04075
Castro-Alba V, Vargas M, Sandberg A-S, Perez-Rea D, Bergenståhl B, Granfeldt Y, et al. Fermented quinoa and canihua in plant‐based diets increase iron and zinc bioavailability in growing rats. Food Sci Nutr [Internet]. 2024;12(11):9555–65. Disponible en: http://dx.doi.org/10.1002/fsn3.4514
Cossio-Bolaños MA, Gómez R, Arruda M, Hochmuller R. Valores de confiabilidad de indicadores somáticos en ratas machos wistar. Actualización Nutr. 2010;296–302.
Cano-Rabano M, Rios-Granja M. Cuidado y mantenimiento de los animales de experimentación. In: Perez-García C, Diéz-Prieto M, García-Partida, P. Introducción a la Experimentación y Protección Animal. Ed. Universidad de León, España 1999, 91-102.
Guide for the care and use of laboratory animals: Eighth edition. Washington, D.C.: National Academies Press; 2011.Tang N, Chen L-Q, Zhuang H. Effects of heme iron enriched peptide on iron deficiency anemia in rats. Food Funct [Internet]. 2014;5(2):390–9. Disponible en: http://dx.doi.org/10.1039/c3fo60292c
Tang N, Chen L-Q, Zhuang H. Effects of heme iron enriched peptide on iron deficiency anemia in rats. Food Funct [Internet]. 2014;5(2):390–9. Disponible en: http://dx.doi.org/10.1039/c3fo60292c
Yang J, Li Q, Feng Y, Zeng Y. Iron deficiency and iron deficiency anemia: Potential risk factors in bone loss. Int J Mol Sci [Internet]. 2023;24(8):6891. Disponible en: http://dx.doi.org/10.3390/ijms24086891
Abrams SA. New approaches to iron fortification: role of bioavailability studies. Am J Clin Nutr [Internet]. 2004;80(5):1104–5. Disponible en: http://dx.doi.org/10.1093/ajcn/80.5.1104
Hurrell RF. Fortification: Overcoming technical and practical barriers. J Nutr [Internet]. 2002;132(4):806S-812S. Disponible en: http://dx.doi.org/10.1093/jn/132.4.806s
Assan N. Socio-cultural, economic, and environmental implications for innovation in sustainable food in Africa. Front Sustain Food Syst [Internet]. 2023;7. Disponible en: http://dx.doi.org/10.3389/fsufs.2023.1192422
Moscoso-Mujica G, Mujica Á, Chávez J, Peña C, Begazo N, Estrella J, et al. Antianemic activity of quinoa (Chenopodium quinoa Willd) Collana Negra variety and kanihua (Chenopodium pallidicaule Aellen) Ramis variety seed flour in anemic rats. SN Appl Sci [Internet]. 2022;4(11):318. Disponible en: http://dx.doi.org/10.1007/s42452-022-05202-w
Rojas, J., Ren, G., Mujica, A. Quinoa: the sacred grain of the Incas. Beltran Publisher. 2022.
Downloads
Published
How to Cite
Issue
Section
Categories
License
Copyright (c) 2025 Nutrición Clínica y Dietética Hospitalaria
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Los lectores pueden utilizar los textos publicados de acuerdo con la definición BOAI (Budapest Open Access Initiative)
