Green algae Caulerpa racemosa inhibit proliferation and promote apoptosis in human HT-29 colon cancer by suppressing PI3K/AKT pathway.
DOI:
https://doi.org/10.12873/443nurkolisKeywords:
Caulerpa racemosa, sea grapes, HT-29, colon cancer, PI3K/AKT, proliferation, apoptosisAbstract
Introduction: Colon cancer is a malignancy of the gas
trointestinal which is one of the most common causes of death worldwide. Tumorigenesis involved oncologic pathway such as PI3K/AKT as the common pathway with the challenges is ther apy resistance. Therefore, an alternative colon cancer treat ments is needed that comes from biological resources such as sea grapes.
Objective: This study aims to determine anticancer po
tency of hexane extract from sea grapes (Caulerpa race
mosa) on HT-29 cell line colon cancer based on suppress
ing PI3K/AKT pathway.
Method: The study was conducted in vitro using the ex
tract of Caulerpa racemosa at concentrations of (0, 400, 800,
and 1.200) µg/mL on the HT-29 cell line based on the level
expression of p-akt related to PI3K/AKT pathway, cleaved
caspase 3 related to apoptosis, and viable cells related to proliferation.
Results: The research results was found that the hexane
extract of Caulerpa racemosa decreased the expression
level of p-akt (Kruskal wallis, p=0,027), increased the
cleaved caspase 3 (Kruskal wallis, p=0,016), and decreased the viable cells (Kruskal wallis, 24 and 48 hours: p=0,024
and 0,034).
Conclusion: Caulerpa racemosa hexane extract can inhibit
colon cancer cells growth by suppressing PI3K/AKT pathway
based on inhibition proliferation and induce apoptosis.
References
Mattiuzzi C, Sanchis-Gomar F, Lippi G. Concise update on colorectal cancer epidemiology. Annals of translational medicine. 2019 Nov;7(21). doi: 10.21037/atm.2019.07.91
Arnold M, Sierra MS, Laversanne M, Soerjomataram I, Jemal A, Bray F. Global patterns and trends in colorectal cancer incidence and mortality. Gut. 2017 Apr 1;66(4):683-91. doi: 10.1136/gutjnl-2015-310912
Mattiuzzi C, Lippi G. Current cancer epidemiology. Journal of epidemiology and global health. 2019 Dec;9(4):217. doi: 10.2991/jegh.k.191008.001
Slattery ML, Mullany LE, Sakoda LC, Wolff RK, Stevens JR, Samowitz WS, Herrick JS. The PI3K/AKT signaling pathway: associations of miRNAs with dysregulated gene expression in colorectal cancer. Molecular carcinogenesis. 2018 Feb;57(2):243-61. doi: 10.1002/mc.22752
Danielsen SA, Eide PW, Nesbakken A, Guren T, Leithe E, Lothe RA. Portrait of the PI3K/AKT pathway in colorectal cancer. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer. 2015 Jan 1;1855(1):104-21. doi: 10.1016/j.bbcan.2014.09.008
Samsarga GW, Affandi Y, Utami NM, Nugraha IM, Manuaba IB. Negative Perceptions of Breast and Colorectal Cancer Patients Against Chemotherapy and Radiotherapy at Hospitals in Denpasar City, Bali. E-Jurnal Medika Udayana. 2014;3(1).
Marfuah I, Dewi EN, Rianingsih L. Study of the potential of sea grape extract (Caulerpa racemosa) as antibacterial against Escherichia coli and Staphylococcus aureus. J Pengolahan dan Bioteknologi Hasil Perikanan. 2018;7(01):7-14.
Dissanayake IH, Bandaranayake U, Keerthirathna LR, Manawadu C, Silva RM, Mohamed B, Ali R, Peiris DC. Integration of in vitro and in-silico analysis of Caulerpa racemosa against antioxidant, antidiabetic, and anticancer activities. Scientific Reports. 2022 Dec 2;12(1):20848. doi: 10.1038/s41598-022-24021-y
Manikandakrishnan M, Palanisamy S, Vinosha M, Kalanjiaraja B, Mohandoss S, Manikandan R, Tabarsa M, You S, Prabhu NM. Facile green route synthesis of gold nanoparticles using Caulerpa racemosa for biomedical applications. Journal of Drug Delivery Science and Technology. 2019 Dec 1;54:101345.
Mert-Ozupek N, Calibasi-Kocal G, Olgun N, Basbinar Y, Cavas L, Ellidokuz H. An Efficient and Quick Analytical Method for the Quantification of an Algal Alkaloid Caulerpin Showed In-Vitro Anticancer Activity against Colorectal Cancer. Marine Drugs. 2022 Nov 30;20(12):757. doi: 10.3390/md20120757
Kerschbaum HH, Tasa BA, Schürz M, Oberascher K, Bresgen N. Trypan blue—Adapting a dye used for labelling dead cells to visualize pinocytosis in viable cells. Cell. Physiol. Biochem. 2021 Jun 1;55:171-84. doi: 10.33594/000000380
Yang H, Liu JX, Shang HX, Lin S, Zhao JY, Lin JM. Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways. World Journal of Gastrointestinal Oncology. 2019 May 5;11(5):377. doi: 10.4251/wjgo.v11.i5.377
Wei R, Xiao Y, Song Y, Yuan H, Luo J, Xu W. FAT4 regulates the EMT and autophagy in colorectal cancer cells in part via the PI3K-AKT signaling axis. Journal of Experimental & Clinical Cancer Research. 2019 Dec;38(1):1-4. doi: 10.1186/s13046-019-1043-0
Manandhar B, Kim HJ, Rhyu DY. Caulerpa okamurae extract attenuates inflammatory interaction, regulates glucose metabolism and increases insulin sensitivity in 3T3-L1 adipocytes and RAW 264.7 macrophages. Journal of Integrative Medicine. 2020 May 1;18(3):253-64. doi: 10.1016/j.joim.2020.02.001
Pradhan B, Patra S, Nayak R, Behera C, Dash SR, Nayak S, Sahu BB, Bhutia SK, Jena M. Multifunctional role of fucoidan, sulfated polysaccharides in human health and disease: A journey under the sea in pursuit of potent therapeutic agents. International Journal of Biological Macromolecules. 2020 Dec 1;164:4263-78. doi: 10.1016/j.ijbiomac.2020.09.019
Ferdous UT, Yusof ZN. Algal terpenoids: A potential source of antioxidants for cancer therapy. Terpenes and Terpenoids-Recent Advances. 2021 Jan 5:63-76.
Jiang M, Qi L, Li L, Li Y. The caspase-3/GSDME signal pathway as a switch between apoptosis and pyroptosis in cancer. Cell death discovery. 2020 Oct 28;6(1):112. doi: 10.1038/s41420-020-00349-0
Li ZL, Mi J, Lu L, Luo Q, Liu X, Yan YM, Jin B, Cao YL, Zeng XX, Ran LW. The main anthocyanin monomer of Lycium ruthenicum Murray induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase 3 signaling pathway in prostate cancer DU-145 cells. Food & Function. 2021;12(4):1818-28. doi: 10.1039/d0fo02382e
Downloads
Published
How to Cite
License
Copyright (c) 2024 Nutrición Clínica y Dietética Hospitalaria
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Los lectores pueden utilizar los textos publicados de acuerdo con la definición BOAI (Budapest Open Access Initiative)
